mouse anti il 1β il f2  (R&D Systems)

Journal: Saudi Pharmaceutical Journal : SPJ

Article Title: Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2

doi: 10.1016/j.jsps.2024.102110

Figure Lengend Snippet: List of forward and reverse primers used in real time PCR assay.

Article Snippet: The membrane was probed with mouse anti-human primary antibodies against TGF-β (Cat.: sc-130348), IL-1β (Cat.: sc-12742), VEGF (Cat.: sc-53462), MMP-2 (Cat.: sc-13595) and MMP-9 (Cat.: sc-21733) (1:500) (Santa Cruz, CA, USA) at 4 °C for 24 h. After the incubation period, the membrane was washed three times with TBST and incubated with anti-mouse horseradish peroxidase-conjugated secondary antibodies (1:1000) (Santacruz, CA, USA, Cat.: sc-2005).

Techniques: Real-time Polymerase Chain Reaction

Journal: Saudi Pharmaceutical Journal : SPJ

Article Title: Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2

doi: 10.1016/j.jsps.2024.102110

Figure Lengend Snippet: Relative mRNA expression of A. TGF-β, B. IL-1β, C. VEGF, and D. MMP-2 genes in HaCaT treated with BFLE. Allantoin is used as a positive control. Results were expressed as mean ± SD expression of respective genes relative to untreated control (NC). ns; not significance, ** P < 0.01, *** P < 0.001 vs control group, n = 3 in each group.

Article Snippet: The membrane was probed with mouse anti-human primary antibodies against TGF-β (Cat.: sc-130348), IL-1β (Cat.: sc-12742), VEGF (Cat.: sc-53462), MMP-2 (Cat.: sc-13595) and MMP-9 (Cat.: sc-21733) (1:500) (Santa Cruz, CA, USA) at 4 °C for 24 h. After the incubation period, the membrane was washed three times with TBST and incubated with anti-mouse horseradish peroxidase-conjugated secondary antibodies (1:1000) (Santacruz, CA, USA, Cat.: sc-2005).

Techniques: Expressing, Positive Control, Control

Journal: Saudi Pharmaceutical Journal : SPJ

Article Title: Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2

doi: 10.1016/j.jsps.2024.102110

Figure Lengend Snippet: Relative protein expression of A. TGF-β, B. IL-1β, C. VEGF and D. MMP-2 protein in HaCaT treated with BFLE. Allantoin is used as a positive control. Results were expressed as mean ± SD expression of respective proteins relative to untreated control (NC). ns; not significance, ** P < 0.01, *** P < 0.001 vs normal control (NC) group, n = 3 in each group.

Article Snippet: The membrane was probed with mouse anti-human primary antibodies against TGF-β (Cat.: sc-130348), IL-1β (Cat.: sc-12742), VEGF (Cat.: sc-53462), MMP-2 (Cat.: sc-13595) and MMP-9 (Cat.: sc-21733) (1:500) (Santa Cruz, CA, USA) at 4 °C for 24 h. After the incubation period, the membrane was washed three times with TBST and incubated with anti-mouse horseradish peroxidase-conjugated secondary antibodies (1:1000) (Santacruz, CA, USA, Cat.: sc-2005).

Techniques: Expressing, Positive Control, Control